iscript cdna 116 synthesis kit Search Results


growth medium  (Cell Applications Inc)
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Cell Applications Inc growth medium
Growth Medium, supplied by Cell Applications Inc, used in various techniques. Bioz Stars score: 96/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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vwr extra pure  (Chem Impex International)
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Chem Impex International vwr extra pure
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cell line nucleofector kit v  (Amaxa)
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Amaxa cell line nucleofector kit v
Cell Line Nucleofector Kit V, supplied by Amaxa, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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human lambda light chain kit  (Bethyl)
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Bethyl human lambda light chain kit
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rneasy kit  (Qiagen)
96
Qiagen rneasy kit
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microrna reverse transcription kit  (Thermo Fisher)
90
Thermo Fisher microrna reverse transcription kit
Microrna Reverse Transcription Kit, supplied by Thermo Fisher, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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tumor associated fibroblast isolation kit  (Miltenyi Biotec)
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Miltenyi Biotec tumor associated fibroblast isolation kit
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fectopro transfection kit  (Sartorius AG)
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Sartorius AG fectopro transfection kit
Fectopro Transfection Kit, supplied by Sartorius AG, used in various techniques. Bioz Stars score: 93/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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plasmid 116 midi kit  (Qiagen)
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Qiagen plasmid 116 midi kit
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maxima h minus first strand cdna synthesis kit  (Thermo Fisher)
90
Thermo Fisher maxima h minus first strand cdna synthesis kit
Maxima H Minus First Strand Cdna Synthesis Kit, supplied by Thermo Fisher, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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genomic mini kit  (A&A Biotechnology)
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A&A Biotechnology genomic mini kit
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qubit dsdna br assay kit  (Thermo Fisher)
90
Thermo Fisher qubit dsdna br assay kit
Correlation plots for DNA quality (as stock concentration and mean fragment size), call rate (CR; the proportion of successfully genotyped SNPs per sample), and age of the Dracocephalum ruyschiana samples (given as collection year). The orange lines represent the average overall historic samples and the orange zones the 95% confidence interval. Each symbol represents an individual sample, the shape of its geographical origin, and the color of its mean DNA fragment size (bp) based on gel electrophoresis (white = no data). (a) DNA quality: Correlation between specimen collection year and <t>Qubit</t> DNA concentration for 113 historical D. ruyschiana specimens. (b) SNP genotyping performance: correlation between specimen collection year and CR for 127 historical and 355 modern D. ruyschiana specimens, displayed to the left and right of the stippled line, respectively. The pink line represents the average over both historical and modern samples and the pink zone is 95% confidence interval. (c) Correlation between Qubit DNA stock concentration and CR for 116 historical D. ruyschiana specimens.
Qubit Dsdna Br Assay Kit, supplied by Thermo Fisher, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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Image Search Results


Correlation plots for DNA quality (as stock concentration and mean fragment size), call rate (CR; the proportion of successfully genotyped SNPs per sample), and age of the Dracocephalum ruyschiana samples (given as collection year). The orange lines represent the average overall historic samples and the orange zones the 95% confidence interval. Each symbol represents an individual sample, the shape of its geographical origin, and the color of its mean DNA fragment size (bp) based on gel electrophoresis (white = no data). (a) DNA quality: Correlation between specimen collection year and Qubit DNA concentration for 113 historical D. ruyschiana specimens. (b) SNP genotyping performance: correlation between specimen collection year and CR for 127 historical and 355 modern D. ruyschiana specimens, displayed to the left and right of the stippled line, respectively. The pink line represents the average over both historical and modern samples and the pink zone is 95% confidence interval. (c) Correlation between Qubit DNA stock concentration and CR for 116 historical D. ruyschiana specimens.

Journal: Ecology and Evolution

Article Title: Spatiotemporal monitoring of the rare northern dragonhead ( Dracocephalum ruyschiana , Lamiaceae) — SNP genotyping and environmental niche modeling herbarium specimens

doi: 10.1002/ece3.9187

Figure Lengend Snippet: Correlation plots for DNA quality (as stock concentration and mean fragment size), call rate (CR; the proportion of successfully genotyped SNPs per sample), and age of the Dracocephalum ruyschiana samples (given as collection year). The orange lines represent the average overall historic samples and the orange zones the 95% confidence interval. Each symbol represents an individual sample, the shape of its geographical origin, and the color of its mean DNA fragment size (bp) based on gel electrophoresis (white = no data). (a) DNA quality: Correlation between specimen collection year and Qubit DNA concentration for 113 historical D. ruyschiana specimens. (b) SNP genotyping performance: correlation between specimen collection year and CR for 127 historical and 355 modern D. ruyschiana specimens, displayed to the left and right of the stippled line, respectively. The pink line represents the average over both historical and modern samples and the pink zone is 95% confidence interval. (c) Correlation between Qubit DNA stock concentration and CR for 116 historical D. ruyschiana specimens.

Article Snippet: We measured DNA yield for 116 of the 130 herbarium samples using the Qubit dsDNA BR Assay Kit (Thermo Fisher Scientific, MA, USA), following the manufacturer's protocol.

Techniques: Concentration Assay, Nucleic Acid Electrophoresis